Conclusions Catalase acts as the catalyzing enzyme in the decomposition of hydrogen peroxide. With a Fahrenheit thermometer, you may want to remove potatoes only every 10 or so degrees. Introduction: Metabolism is the sum total of chemical reactions in the body that are necessary to the maintenance of life. Place 2 ml of hydrogen peroxide in each of 3 clean test tubes and then add each of the three test substances to the tubes. The solution should progress in the colors of blue with no glucose present , green, yellow, orange, red, and then brick red when there is a large amount of glucose present.
Hydrogen peroxide is produced naturally in organisms, but is very harmful. After 5 minutes pull out bung, read the measurement on the upside down measuring cylinder. All enzyme catalysed reactions are reversible. Place the pot of potatoes and water onto the stove and turn on low. Using a centrifuge would mean you could use the exact amount of catalase each time. Besides playing an important role in the preservation of life in living organisms, the catalase enzyme also has commercial uses.
Exact amounts do not matter, as long as they are relatively close. This enzyme, like many others, aids in the decomposition of one substance into another. The reaction will increase with the increasing enzyme concentration when the molecules of hydrogen peroxide are freely available. In this experiment, the student will work to determine the temperature at which catalase denatures and is no longer active. I will ensure this by using catalase and hydrogen peroxide from the same batch in each experiment. Substance Apple Potato Yeast Rate of Reaction 0-5 Synthesis -- Do all living tissues contain catalase? The second is the non competitive inhibitor which distorts the shape of the active site. To find a suitable correlation, I should use at least 8 samples.
Since our potato was raw, the catalase in the enzyme of the potato was not altered or damaged at all. The following graph shows reaction rates of various enzymes in the body. Therefore beyond a certain temperature, the rate of reaction will decrease. Also, the reaction is so vigorous that bubbles of mixture can carry pieces of liver into the delivery tube. Hydrogen peroxide is a molecule that can attack sulphur atoms and metal ions in protein molecules, and with the aid of iron ions is potentionally mutagenic. Under the influence of an enzyme called catalase, the hydrogen peroxide is broken down into water and oxygen.
The speed at which this modification occurs relies on two main factors; the amount of collisions between reacting particles and what portion of these collisions are successful in producing a change within the substances present. Replace bung immediately and start stopwatch for 5 minutes. The key is like the substrate that comes and bonds to the active site or the key that fits into the lock. The enzyme would be damaged and not be able to perform the same way. When 30oC, place the potato in the solution, quickly attaching it to the delivery tube and starting the stopclock a helper is advised here.
A large potato can easily weigh around 500g, so the problem then comes with different concentrations of catalase distributed within the potato. The results of this experiment… 3155 Words 13 Pages The Effect of Substrate Concentration on the Rate of Activity of the Enzyme Catalase Background Information: Enzymes are biological catalysts that increase the speed of chemical reactions without undergoing any physical change. Design an experiment where you would determine how quicly lactaid works to break down milk sugar at different temperatures. Step 7: Repeat steps 1-6 for 20mL and 30mL of hydrogen peroxide. The hypothesis was that hydrogen peroxide will be broken down by catalase into hydrogen and oxygen, where a higher concentration of inhibitor will yield less oxygen, resultant of a lower rate of reaction. Stop removing potatoes when they are completely soft, when the water boils, or when the potato is gone, whichever occurs first.
Compare your results to your hypothesis. In this experiment a pH 7 buffer was used. Perform the experiment three times 10. Be specific in your description, use drawings if necessary. The above would be considered the control for the experiment and simply indicated the presence of catalase in the potato. Plan Aim: To investigate the rate of the effect of Catalase on hydrogen peroxide. If using small glasses, use about 5 tablespoons, or fill about an inch deep.
Oxygen gas was produced Describe the test that was performed in order to identify the gas. This is where another molecule s can bind with the enzyme. We conducted the experiment so that we were able to have a control group utilizing water while we were able to change the variables with hydrogen peroxide, ammonia, and vinegar. Yes, you can mash or blend potatoes ahead of time. Potatoes contain catalase, which is an enzyme found in nearly all living organisms exposed to oxygen. It was proposed that when the catalase increases in concentration at the increments of 0.
You can also do this experiment with a potato slice. Lesson organisation You could run this investigation as a demonstration at two different concentrations, or with groups of students each working with a different concentration of hydrogen peroxide. Our results showed that as the concentration of H2O2 increased, the rate of reaction increased. Potato has a very high enzyme count, that¡¦s why we use it in this experiment. I will help you with the equation. They are used to speed up specific reactions in the cells.